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KMID : 0545119950050050264
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Journal of Microbiology and Biotechnology
1995 Volume.5 No. 5 p.264 ~ p.268
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Screening for Inhibitor of c-myc Expression and Identification of Isolate No.2303
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Chung, Ji-Hyung
Yeo, Ick-Hyun/Oh, Doo-Whan/Moon, Soon-Ok
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Abstract
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Sulforhodamine B(SRB) assay was performed on the human lung carcinoma, A549 cell line to screen soil microorganisms for production of anti-cancer agent. Among 4,265 microorganisms, 45 isolates were selected for their cytotoxicity and tested for their effects on the expression of c-myc by RNA slot blot and Northern blot analysis resulting in selection of No.2303 isolate. This No.2303 was identified as Streptomyces sp. by ISP classification and the chemotaxonomic analysis method. N0.2303 inhibited the expression of cmyc in Colo320 DM and A549 cell lines. The culture extract of No. 2303 also inhibited the progression of the cell cycle of G_o in NIH 3T3 cells, implying that the extract also inhibited the expression of c-myc in NIH 3T3 cell.
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